| Filename | Total | Flagged As Poor Quality | Sequence Length | %GC |
|---|---|---|---|---|
| 1_non_mutant_Q96_K97del_6mth_10_03_2016_S1_fem_R1.fq.gz | 45,225,393 | 0 | 35-150 | 45 |
| 1-MORGAN-6P-PN1_S2_R1_001.fq.gz | 26,290,224 | 0 | 35-76 | 44 |
| 10_Ps2Ex3M1_WT_6month_07_07_2016_F3_86_Fem_R1.fq.gz | 32,598,508 | 0 | 35-150 | 46 |
| 11__-_5_R1.fq.gz | 21,272,369 | 0 | 35-76 | 45 |
| 11_Ps2Ex3M1_WT_6month_07_07_2016_F3_88_Fem_R1.fq.gz | 34,573,446 | 0 | 35-150 | 47 |
| 12__-_4_R1.fq.gz | 22,070,458 | 0 | 35-76 | 45 |
| 12_9W_1.fq.gz | 51,361,917 | 0 | 35-150 | 44 |
| 12_Ps2Ex3M1_WT_6month_07_07_2016_F3_95_Fem_R1.fq.gz | 3.2e+07 | 0 | 35-150 | 46 |
| 13__-_3_R1.fq.gz | 1.7e+07 | 0 | 35-76 | 45 |
| 14__-_2_R1.fq.gz | 24,266,600 | 0 | 35-76 | 45 |
| 15__-_1_R1.fq.gz | 25,643,654 | 0 | 35-76 | 45 |
| 2_non_mutant_Q96_K97del_6mth_10_03_2016_S2_fem_R1.fq.gz | 39,097,924 | 0 | 35-150 | 44 |
| 2-MORGAN-6P-PN2_S5_R1_001.fq.gz | 45,232,784 | 0 | 35-76 | 44 |
| 3_13W_1.fq.gz | 42,022,566 | 0 | 35-150 | 43 |
| 3_non_mutant_Q96_K97del_6mth_10_03_2016_S3_fem_R1.fq.gz | 42,756,149 | 0 | 35-150 | 44 |
| 3-MORGAN-6P-PN3_S10_R1_001.fq.gz | 29,274,876 | 0 | 35-76 | 44 |
| 4_14W_1.fq.gz | 46,784,129 | 0 | 35-150 | 43 |
| 4_non_mutant_K97Gfs_6mth_10_03_2016_S1_fem_R1.fq.gz | 46,346,616 | 0 | 35-150 | 43 |
| 4-MORGAN-6P-PN4_S8_R1_001.fq.gz | 32,027,574 | 0 | 35-76 | 43 |
| 5_non_mutant_K97Gfs_6mth_10_03_2016_S2_fem_R1.fq.gz | 38,963,892 | 0 | 35-101 | 43 |
| 6_16W_1.fq.gz | 63,383,444 | 0 | 35-150 | 44 |
| 6_non_mutant_K97Gfs_6mth_10_03_2016_S3_fem_R1.fq.gz | 83,022,890 | 0 | 35-150 | 44 |
| 8_19W_1.fq.gz | 30,142,041 | 0 | 35-150 | 43 |
| 9_1W_1.fq.gz | 21,154,240 | 0 | 35-150 | 43 |
| 9_Ps2Ex3M1_WT_6month_07_07_2016_F3_79_Fem_R1.fq.gz | 27,960,314 | 0 | 35-150 | 47 |
| W1_ss.fq.gz | 46,324,895 | 0 | 35-150 | 43 |
| W2_ss.fq.gz | 44,093,591 | 0 | 35-150 | 44 |
| W3_ss.fq.gz | 45,456,094 | 0 | 35-150 | 43 |
| W4_ss.fq.gz | 53,914,220 | 0 | 35-150 | 43 |
| W5_ss.fq.gz | 41,470,209 | 0 | 35-150 | 43 |
Library Sizes ranged between 17,003,150 and 83,022,890 reads.
Read totals for each library. Duplicated reads are conventionally an high overestimate at this point.
Summary of FastQC flags for each parameter
Heatmap showing mean base qualities for each library
Heatmap showing mean sequence qualities for each library
Heatmap of summed base distributions along each read
GC Content Heatmap normalised to theoretical GC content in the Hsapiens Genome
GC Content Distributions for all reads showing theoretical GC content from the Hsapiens Genome
Total overrepresented sequences for each library
## Overrepresented_sequences missing from 1-MORGAN-6P-PN1_S2_R1_001.fq.gz## Overrepresented_sequences missing from 11__-_5_R1.fq.gz## Overrepresented_sequences missing from 12__-_4_R1.fq.gz## Overrepresented_sequences missing from 12_9W_1.fq.gz## Overrepresented_sequences missing from 13__-_3_R1.fq.gz## Overrepresented_sequences missing from 14__-_2_R1.fq.gz## Overrepresented_sequences missing from 15__-_1_R1.fq.gz## Overrepresented_sequences missing from 2-MORGAN-6P-PN2_S5_R1_001.fq.gz## Overrepresented_sequences missing from 3_13W_1.fq.gz## Overrepresented_sequences missing from 3-MORGAN-6P-PN3_S10_R1_001.fq.gz## Overrepresented_sequences missing from 4_14W_1.fq.gz## Overrepresented_sequences missing from 4-MORGAN-6P-PN4_S8_R1_001.fq.gz## Overrepresented_sequences missing from 6_16W_1.fq.gz## Overrepresented_sequences missing from 8_19W_1.fq.gz## Overrepresented_sequences missing from 9_1W_1.fq.gz## Overrepresented_sequences missing from W1_ss.fq.gz## Overrepresented_sequences missing from W2_ss.fq.gz## Overrepresented_sequences missing from W3_ss.fq.gz## Overrepresented_sequences missing from W4_ss.fq.gz## Overrepresented_sequences missing from W5_ss.fq.gz| Sequence | Total | Present In | Overall Percentage | Max_Percentage | Possible_Source |
|---|---|---|---|---|---|
| CCGCTGTATTACTCAGGCTGCACTGCAGTGTCTATTCACAGGCGCGATCC | 1,915,608 | 10 | 0.166% | 0.65% | No Hit |
| CGCTGTATTACTCAGGCTGCACTGCAGTGTCTATTCACAGGCGCGATCCC | 1,673,850 | 6 | 0.145% | 0.66% | No Hit |
| GTGGGTTCAGGTAATTAATTTAAAGCTACTTTCGTGTTTGGGCCTCTAGC | 1,421,432 | 4 | 0.123% | 1.66% | No Hit |
| GGTTCAGGTAATTAATTTAAAGCTACTTTCGTGTTTGGGCCTCTAGCATC | 1,195,842 | 4 | 0.104% | 1.08% | No Hit |
| CTGAAGTCTTGGAGCTCGACTTCCCTACGTTTCGCCTACAAATGTTGCCT | 866,936 | 6 | 0.075% | 0.35% | No Hit |
| GGGGTGTACGAAGCTGAACTTTTATTCATCTCCCAGACAACCAGCTATTG | 719,598 | 4 | 0.062% | 1.03% | No Hit |
| GTTCAGGTAATTAATTTAAAGCTACTTTCGTGTTTGGGCCTCTAGCATCT | 660,825 | 4 | 0.057% | 0.57% | No Hit |
| GGGTTCAGGTAATTAATTTAAAGCTACTTTCGTGTTTGGGCCTCTAGCAT | 645,351 | 4 | 0.056% | 0.76% | No Hit |
| CTTAGACGACCTGGTAGTCCAAGGCTCCCCCAGGAGCACCATATCGATAC | 501,473 | 7 | 0.044% | 0.26% | No Hit |
| CCTCCTTCAAGTATTGTTTCATGTTACATTTTCGTATATTCTGGGGTAGA | 462,364 | 4 | 0.040% | 0.57% | No Hit |
| CTCCTGAAAAGGTTGTATCCTTTGTTAAAGGGGCTGTACCCTCTTTAACT | 438,172 | 3 | 0.038% | 0.69% | No Hit |
| GCTGTATTACTCAGGCTGCACTGCAGTGTCTATTCACAGGCGCGATCCCA | 418,110 | 6 | 0.036% | 0.16% | No Hit |
| CGGGTCGGGTGGGTGGCCGGCATCACCGCGGACCTCGGGCGCCCTTTTGG | 412,467 | 4 | 0.036% | 0.73% | No Hit |
| GGGCCTCTAGCATCTAAAAGCGTATAACAGTTAAAGGGCCGTTTGGCTTT | 406,142 | 3 | 0.035% | 0.59% | No Hit |
| GTATTACTCAGGCTGCACTGCAGTGTCTATTCACAGGCGCGATCCCACTA | 364,611 | 6 | 0.032% | 0.14% | No Hit |
| GGGTGTACGAAGCTGAACTTTTATTCATCTCCCAGACAACCAGCTATTGC | 358,921 | 4 | 0.031% | 0.44% | No Hit |
| CGGGTCGGGTGGGTAGCCGGCATCACCGCGGACCTCGGGCGCCCTTTTGG | 342,333 | 4 | 0.030% | 0.56% | No Hit |
| CCCGCTGTATTACTCAGGCTGCACTGCAGTGTCTATTCACAGGCGCGATC | 315,738 | 4 | 0.027% | 0.39% | No Hit |
| CTCCGACCTGGGCCGGTTCACCCCTCCTTAGACGACCTGGTAGTCCAAGG | 238,609 | 4 | 0.021% | 0.12% | No Hit |
| GGCCTCTAGCATCTAAAAGCGTATAACAGTTAAAGGGCCGTTTGGCTTTA | 219,458 | 3 | 0.019% | 0.37% | No Hit |
| ATTACTCAGGCTGCACTGCAGTGTCTATTCACAGGCGCGATCCCACTACT | 190,314 | 3 | 0.017% | 0.12% | No Hit |
| GGTGTACGAAGCTGAACTTTTATTCATCTCCCAGACAACCAGCTATTGCC | 182,193 | 4 | 0.016% | 0.21% | No Hit |
| AGCTGGGGAGATCCGCGAGAAGGGCCCGGCGCACGTCCAGAGTCGCCGCC | 174,069 | 3 | 0.015% | 0.23% | No Hit |
| GGTCGGGTGGGTGGCCGGCATCACCGCGGACCTCGGGCGCCCTTTTGGAC | 171,124 | 3 | 0.015% | 0.25% | No Hit |
| GTCCCCCACTACCAGAAATTATGCAGTCGAGATTCCCACATTTGGGGAAT | 160,169 | 3 | 0.014% | 0.19% | No Hit |
| GGCCCGGCGCACGTCCAGAGTCGCCGCCGCGCACCGCAGCGCATCCCCCC | 155,419 | 2 | 0.013% | 0.35% | No Hit |
| GTCTGAAGTCTTGGAGCTCGACTTCCCTACGTTTCGCCTACAAATGTTGC | 155,346 | 3 | 0.013% | 0.23% | No Hit |
| GCCCACTACGACAACGTGTTTTGTAAATTATGATCTTTATTCTCCTGAAA | 152,742 | 2 | 0.013% | 0.24% | No Hit |
| GCTCGGGTGTCTACATCCATTCCTACAGTGAATATGTGATGGGCTCATAC | 142,619 | 3 | 0.012% | 0.20% | No Hit |
| TGGGTTCAGGTAATTAATTTAAAGCTACTTTCGTGTTTGGGCCTCTAGCA | 133,783 | 3 | 0.012% | 0.19% | No Hit |
Universal Adapter Content
## Kmer_Content missing from 1_non_mutant_Q96_K97del_6mth_10_03_2016_S1_fem_R1.fq.gz## Kmer_Content missing from 1-MORGAN-6P-PN1_S2_R1_001.fq.gz## Kmer_Content missing from 10_Ps2Ex3M1_WT_6month_07_07_2016_F3_86_Fem_R1.fq.gz## Kmer_Content missing from 11__-_5_R1.fq.gz## Kmer_Content missing from 11_Ps2Ex3M1_WT_6month_07_07_2016_F3_88_Fem_R1.fq.gz## Kmer_Content missing from 12__-_4_R1.fq.gz## Kmer_Content missing from 12_9W_1.fq.gz## Kmer_Content missing from 12_Ps2Ex3M1_WT_6month_07_07_2016_F3_95_Fem_R1.fq.gz## Kmer_Content missing from 13__-_3_R1.fq.gz## Kmer_Content missing from 14__-_2_R1.fq.gz## Kmer_Content missing from 15__-_1_R1.fq.gz## Kmer_Content missing from 2_non_mutant_Q96_K97del_6mth_10_03_2016_S2_fem_R1.fq.gz## Kmer_Content missing from 2-MORGAN-6P-PN2_S5_R1_001.fq.gz## Kmer_Content missing from 3_13W_1.fq.gz## Kmer_Content missing from 3_non_mutant_Q96_K97del_6mth_10_03_2016_S3_fem_R1.fq.gz## Kmer_Content missing from 3-MORGAN-6P-PN3_S10_R1_001.fq.gz## Kmer_Content missing from 4_14W_1.fq.gz## Kmer_Content missing from 4_non_mutant_K97Gfs_6mth_10_03_2016_S1_fem_R1.fq.gz## Kmer_Content missing from 4-MORGAN-6P-PN4_S8_R1_001.fq.gz## Kmer_Content missing from 5_non_mutant_K97Gfs_6mth_10_03_2016_S2_fem_R1.fq.gz## Kmer_Content missing from 6_16W_1.fq.gz## Kmer_Content missing from 6_non_mutant_K97Gfs_6mth_10_03_2016_S3_fem_R1.fq.gz## Kmer_Content missing from 8_19W_1.fq.gz## Kmer_Content missing from 9_1W_1.fq.gz## Kmer_Content missing from 9_Ps2Ex3M1_WT_6month_07_07_2016_F3_79_Fem_R1.fq.gz## Kmer_Content missing from W1_ss.fq.gz## Kmer_Content missing from W2_ss.fq.gz## Kmer_Content missing from W3_ss.fq.gz## Kmer_Content missing from W4_ss.fq.gz## Kmer_Content missing from W5_ss.fq.gz## No Kmers were detected by FastQC